FIG. 5.

C. rodentium associates with crypts that are strongly positive for Muc2 at day 10 p.i. (A) Dual immunofluorescence staining for C. rodentium Tir (red) and Muc2 (green) in a distal colon at day 10 p.i. Tir staining (white arrow) is associated with crypts strongly positive for Muc2 (white arrowhead), but little if any Tir staining (yellow arrow) is associated with crypts that are weakly positive for Muc2 (yellow arrowhead). Note the frequent colocalization of Tir with Muc2-postive cells and luminal Muc2. (B) Proportions of crypts exhibiting depletion of Muc2-positive goblet cells that are positive for Tir compared to crypts that are not depleted of Muc2-postive goblet cells. The bars indicate the averages for 30 crypts counted within colonic sections from two individual mice, and the error bars indicate standard deviations.