Figure 3.

PR-mediated chromatin remodeling is ligand-dependent. Where indicated, PR_B (6 pmol) and progesterone (10⁻⁷ M) were added to preassembled chromatin (200 ng). After 30-min incubation, the chromatin templates were subjected to micrococcal nuclease digestion. Reaction mixtures shown in each set of three were incubated with micrococcal nuclease (4 units) for 1, 5, and 10 min at room temperature, respectively. Blots were hybridized to a distal probe (located ≈900 bp upstream of transcription initiation site) to assess the regularity of the nucleosomal array in bulk chromatin (Left), and with a promoter probe (corresponding to the PRE sequence) to analyze the disruption in nucleosome array near the PR-binding sites (Right).