FIG. 4.

Localization of Rcp proteins. Subcellular fractions of CU1000N (wild type [wt]) and Aa0727, the tadD mutant strain with added TadD-T7 (via pJMM108), were prepared by differential detergent solubilization of the inner membrane with 0.5% Sarkosyl, as described in the text. Immunoblots were probed with antiserum specific to RcpC, RcpA, RcpB, or TadC or to the T7 epitope tag. Arrows indicate the positions of RcpC, RcpA, RcpB, TadC, and TadD-T7. S, soluble fraction; IM, inner membrane; OM, outer membrane.