TABLE 4.
Kinetic constants of Ndx2 mutantsa
Mutation | Substrate | Km (μM) | kcat (s−1) | kcat/Km (M−1 s−1) |
---|---|---|---|---|
None (wild type) | ADPR | 520 | 1.4 | 2,700 |
FAD | 300 | 2.5 | 8,300 | |
E136Q | ADPR | 660 | 0.12 | 180 |
FAD | 400 | 0.27 | 680 | |
E90Q | ADPR | 820 | 0.00022 | 0.27 |
FAD | 640 | 0.00067 | 1.0 | |
E94Q | ADPR | 1000 | 0.00014 | 0.14 |
FAD | 790 | 0.00017 | 0.21 |
Assays were done as described in Materials and Methods.