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. 2007 Nov 30;190(3):946–953. doi: 10.1128/JB.01571-06

FIG. 5.

FIG. 5.

Determination of the half-life of RpoS during bioreactor growth of A. vinelandii in BBGN and BBG medium. (A) Relative RpoS expression during an acetate-to-glucose diauxic shift. Samples were removed during acetate limitation (i), diauxic lag (ii), and growth recovery on glucose (iii) and then treated with tetracycline to stop translational initiation. (B) Western analysis of RpoS following tetracycline addition. (C) Relative amount of RpoS following treatment plotted as a percentage of the RpoS present prior to tetracycline addition. The calculated half lives are indicated in panel A. (D and E) RpoS expression during growth on BBG medium. Samples were removed at two time points (iv and v) and treated with tetracycline. The relative amount of RpoS following treatment was plotted as a percentage of the RpoS present prior to tetracycline addition (E), and the calculated half-lives are indicated in panel D.