FIG. 4.

Binding of H-NS and LeuO to the regulatory region of the yjjP-yjjQ-bglJ locus. (A) Schematic representation of the intergenic region. Regions bound by H-NS and LeuO, as deduced from the results shown in panels B to D, are indicated by a black polygon (LeuO) and gray circles forming an extended complex (H-NS). The positions of the fragments used in the EMSA are schematically indicated (with the specific positions given in Fig. 3). n.t., not tested. (B) Binding of H-NS. Fragments 1 (243 bp) and 2 (170 bp) and the 360-bp lacZ control fragment “C” as well as fragments 3 (140 bp) and 4 (200 bp) and the 360-bp lacZ control were mixed and incubated with the indicated amounts of H-NS. Subsequently, the samples were separated on native acrylamide gels stained with ethidium bromide. (C) Binding of LeuO-His₆ to fragments 1 to 4 in comparison to the binding of the 360-bp control fragment “C.” (D) Binding of LeuO to fragments 1.1 to 1.4 (75 bp each) in comparison to that of the 140-bp lacZ control fragment “C.”