TABLE 1.
V. parahaemolyticus strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Parent; reference |
|---|---|---|
| Strains | ||
| LM1017 | flgE313L::lux; lux fusion in lateral flagellar hook gene (translucent cell type) | BB22; 30 |
| LM5435 | LM1017/pLM2796 (scrABC+) | 7 |
| LM5674 | ΔopaR1 (translucent cell type) | 15 |
| LM5818 | cpsA1::lacZ ΔopaR1 | LM5674; 17 |
| LM5949 | flgCL3061::lacZ (Camr) ΔopaR1 | LM5674; 22 |
| LM6349 | LM1017/pLM1877 | |
| LM6453 | LM5818/pLM1877 | |
| LM6565 | ΔscrABC4::Cam flgE313L::lux | LM1017 |
| LM6567 | ΔscrABC4::Cam | LM5674 |
| LM6832 | ΔscrABC4::Cam cpsA1::lacZ ΔopaR1 | LM5818 |
| LM6837 | LM5818/pLM2796 (scrABC+) | |
| LM6933 | LM6565/pLM2449 (scrC+) | |
| LM6954 | LM5818/pLM3276 (scrCΔEAL) | |
| LM6998 | ΔscrC3::Cam flgE313L::lux | LM1017 |
| LM6999 | ΔscrC3::Cam cpsA1::lacZ ΔopaR1 | LM5818 |
| LM7001 | scrCΔEAL::Cam flgE313L::lux | LM1017 |
| LM7002 | scrCΔEAL::Cam cpsA1::lacZ ΔopaR1 | LM5818 |
| LM7012 | LM5818/pLM2449 (scrC+) | |
| LM7053 | LM1017/pLM2449 (scrC+) | |
| LM7054 | LM1017/pLM3276 (scrCΔEAL) | |
| LM7074 | LM6565/pLM1877 | |
| LM7665 | LM6565/pLM3437 (scrABCΔEAL) | |
| LM7667 | LM6565/pLM2796 (scrABC+) | |
| LM8021 | ΔscrC3::Cam ΔopaR1 | LM5674 |
| LM8022 | scrCΔEAL::Cam ΔopaR1 | LM5674 |
| LM8078 | LM1017/pLM3437 (scrABCΔEAL) | |
| LM8197 | LM5818/pLM3437 (scrABCΔEAL) | |
| LM8309 | LM1017/pLM3598 (scrABCE554A) | |
| LM8310 | LM1017/pLM3599 (scrABCD428A) | |
| LM8311 | LM5818/pLM3598 (scrABCE554A) | |
| LM8312 | LM5818/pLM3599 (scrABCD428A) | |
| LM8400 | LM1017/pLM3596 (scrCE554A) | |
| LM8401 | LM1017/pLM3597 (scrCD428A) | |
| LM8403 | LM5818/pLM3596 (scrCE554A) | |
| LM8404 | LM5818/pLM3597 (scrCD428A) | |
| LM8602 | flgCL3061::lacZ (Camr) ΔopaR1 scrC292::Tn5lux (Kanr; lux aligned) | LM5949 |
| LM8604 | flgCL3061::lacZ (Camr) ΔopaR1 scrC803::Tn5lux (Kanr; lux not aligned) | LM5949 |
| LM9000 | LM5674/pLM2641 (gfp+) | |
| LM9001 | LM6567/pLM2641 (gfp+) | |
| LM9002 | LM8022/pLM2641 (gfp+) | |
| Plasmids | ||
| pKD3 | Camr template plasmid | 12 |
| pLM1877 | Genr expression vector | 6 |
| pLM2032 | Tetr cosmid containing scrABC locus | 7 |
| pLM2449 | Genr IPTG-inducible scrC+ | pLM1877; 7 |
| pLM2641 | Genr IPTG-inducible sgGFP | pLM1877; 14 |
| pLM2796 | Genr IPTG-inducible scrABC+ | 7 |
| pLM3272 | Cosmid pLM2032 with ΔscrC3::Cam | 7 |
| pLM3273 | Cosmid pLM2032 with scrCΔEAL::Cam | LM2032 |
| pLM3276 | Genr IPTG-inducible scrCΔEAL | LM2449 |
| pLM3437 | Genr IPTG-inducible scrABCΔEAL | LM2796 |
| pLM3596 | Genr IPTG-inducible scrCE554A | LM2449 |
| pLM3597 | Genr IPTG-inducible scrCD428A | LM2449 |
| pLM3598 | Genr IPTG-inducible scrABCE554A | LM2796 |
| pLM3599 | Genr IPTG-inducible scrABCD428A | LM2796 |