TABLE 3.
Sequencing results and mutations in thyA in clinical normal strains and TD-SCVs
| Strain | Alteration(s) in nucleotide sequence | Predicted results |
|---|---|---|
| Normal-1a | ||
| SCV-1a | Δ247AAT249 | 3-bp deletion → deletion of Asn |
| Normal-2a | ||
| SCV-2a | Δ247AAT249 | 3-bp deletion → deletion of Asn |
| Normal-3b | T76C | Point mutation Cys → Arg (amino acid 26) |
| SCV-3b | C139T, G337A, A493G, ΔA565 | Point mutations Leu → Por (amino acid 47), Gly → Ser (amino acid 113), and Asn → Asp (amino acid 165) and 1-bp deletion → stop codon (amino acid 188) |
| Normal-4a | T730G, G835A | Point mutations Ser → Ala (amino acid 244) and Gly → Ser (amino acid 279) |
| SCV-4a | T82A, Δ592CTTCCGCCTTG601 | 11-bp deletion (amino acid 198 → frameshift mutation → truncated protein |
| Normal-5b | G308A, C838T | Point mutations Gly → Asp (amino acid 103) and Leu → Phe (amino acid 280) |
| SCV-5 | ΔA51 | 1-bp deletion → frameshift mutation → stop codon (amino acid 19) |
| Normal-6b | C383T, A482G, G886A | Point-mutations Ser → Phe (amino acid 128), Asp → Gly (amino acid 161), and Val → Ile (amino acid 296) |
| SCV-6b | G359A, ΔG743 | Point-mutation Arg → Gln (amino acid 120) and 1-bp deletion → frameshift mutation → stop codon (amino acid 251) |
| SCV-7-1b | T7A, C521T, G806A, ΔA893 | Point-mutations Tyr → Asn (amino acid 3), Tyr → Ile (amino acid 174), and Gly → Asp (amino acid 269) and 1-bp deletion → frameshift mutation → stop codon (amino acid 311) |
| SCV-7-2b | T7A, T616C | Point-mutation Tyr → Asn (amino acid 3) and point mutation → stop codon (amino acid 206) |
| SCV-8-1 | ΔT121 | 1-bp deletion → frameshift mutation → stop codon (amino acid 50) |
| SCV-8-2b | Δ547ACATCGT553 | 7-bp deletion → frameshift mutation → stop codon (amino acid 212) |
a
Allele 2 (C at nucleotide position 300, Asn at amino acid position 100, and same thyA sequence as S. aureus strain MW2).
b
Allele 1 (A at nucleotide position 300, Lys at amino acid position, and same thyA sequence as S. aureus strain COL).