FIG. 1.

Coomassie blue-stained SDS-7% polyacrylamide gel (A and C) and immunoblot (B) demonstrating reactivity of MAb 6E3 to a high-molecular-weight antigen in A. baumannii 307-0294 whole-cell lysate (lane 1) and outer membrane proteins (lane 3) but not the corresponding samples from A. baumannii bap1302::EZ-Tn5 (lanes 2 and 4, respectively). (C) Enlargement of the region outlined in panel A to clearly show the high-molecular-weight antigen present in wild type and absent in A. baumannii bap1302::EZ-Tn5 whole-cell lysate. This band was excised from a similar gel and identified by NanoLC-MS/MS. Molecular mass standards are indicated in kilodaltons.