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Flow cytometric analysis of A. baumannii 307-0294 (A) and bap1302::EZ-Tn5 (B) demonstrates the surface accessibility of the MAb 6E3 epitope. Cells from late-log-phase cultures were probed with Alexa Fluor 488-conjugated control nonspecific antibody (shaded histograms) or MAb 6E3 (unshaded histograms). Each histogram shows the fluorescence intensity distribution of >50,000 flow cytometer events.
