FIG. 5.

RT-PCR and agarose gel electrophoresis demonstrates that bap and A1S_2695 are not cotranscribed and that transcription of A1S_2695 is not disrupted in A. baumannii bap1302::EZ-Tn5. (A) Each primer set amplifies a product from chromosomal DNA template. Pr1160-1330 (lane 3), however, fails to amplify a product from RNA template (C), indicating that bap and A1S_2695 are not present on the same fragment of RNA. The samples in lane 5 included RNA template but were not subject to the RT step, as a control for DNA contamination, and hence were not applicable to the reaction sets containing either chromosomal DNA (A) or no nucleic acid template (B). Primers (Table 1) were designed to target the indicated regions: lane 1, 5′ bap; lane 2, 3′ bap; lane 3, 3′ bap-A1S_2695; lane 4, A1S_2695; and lane 5, 3′ bap (no template control).