FIG. 6.

CLSM images of biofilms formed by A. baumannii 307-0294 (A) and bap1302::EZ-Tn5 (B) clearly demonstrate the decreased thickness achieved by the mutant after 48 h in static culture. Biofilms were grown in 35 mm glass-bottom petri dishes for 48 h and stained with the LIVE/DEAD BacLight bacterial viability kit, which stains live cells green with SYTO-9 and dead cells red with propidium iodide. The images show the average intensity projections through the confocal image stack, with the maximum intensity x-z and y-z projections shown along the bottom and side of each image. Images were produced in ImageJ.