FIG. 7.
Comparison of biovolume (A) and mean biofilm thickness (B) achieved by A. baumannii 307-0294 (▪) and A. baumannii bap1302::EZ-Tn5 (▴) during static culture. The data points are the means ± the standard deviations for two separate high-power fields of view, representing a total slide surface area of 105 μm2 per sample. The best-fit nonlinear regression curve is shown as a thin trend line with 95% confidence bands. Because the biofilms formed by wild-type and mutant bacteria cannot be described with the same curve (P < 0.0001), we conclude that the biofilms differ significantly with respect to biovolume and mean biofilm thickness development over time.