TABLE 2.
Levels of polyamines in CAG2242/pUCmdtJI and its mutants cultured with 2 mM spermidinea
| Strain | Polyamine concn (mM)
|
|
|---|---|---|
| Putrescine | Spermidine | |
| CAG2242/pUCmdtJI | 0.49 ± 0.12 | 11.7 ± 0.42 |
| mdtJ(Y4L) | 0.14 ± 0.05 | 36.0 ± 1.39 |
| mdtJ(W5L) | 0.23 ± 0.07 | 24.1 ± 0.99 |
| mdtJ(E15Q) | 0.07 ± 0.03 | 24.7 ± 1.83 |
| mdtJ(Y45L) | 0.33 ± 0.14 | 31.1 ± 0.64 |
| mdtJ(Y61L) | 0.36 ± 0.09 | 33.0 ± 1.56 |
| mdtJ(E82Q) | 0.19 ± 0.11 | 31.9 ± 2.01 |
| mdtI(E5Q) | 0.36 ± 0.18 | 30.9 ± 2.30 |
| mdtI(E19Q) | 0.60 ± 0.24 | 23.2 ± 1.35 |
| mdtI(D60N) | 0.65 ± 0.15 | 20.9 ± 0.49 |
| mdtI(W68L) | 0.24 ± 0.10 | 29.4 ± 2.14 |
| mdtI(W81L) | 0.18 ± 0.13 | 20.7 ± 0.88 |
a
E. coli CAG2242/pUCmdtJI and its mutants were cultured in the presence of 2 mM spermidine for 24 h, and the polyamine content in cells was measured as described in Materials and Methods. Values are means ± SD for three samples.