TABLE 2.
Qualitative capillary assay results with E. coli wild type and MCP deletion mutants
| Chemical stimulus (concn) | RP437 (wild-type) response | Mutant (deletion) chemotaxis response
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| RP1131 (Δtrg) | RP2361 (Δtar) | RP3525 (Δtap) | RP5700 (Δtsr ) | UU1250 (Δ[tar-tap] ΔtsrΔtrg ) | UU1615 (Δ[tar−tap]Δtrg) | UU1624 (Δtsr Δtrg Δtap) | UU1625 (Δtsr Δtrg ) | ||
| None (buffer only) | − | − | − | − | − | − | − | − | − |
| CAA (2%)a | + | + | + | + | + | − | + | + | + |
| Aspartate (10−3 M) | + | + | − | + | + | − | − | + | + |
| Thymine (2 × 10−2 M) | + | + | + | − | + | − | − | − | + |
| Uracil (2 × 10−2 M) | + | + | + | − | + | − | − | − | + |
a
CAA, Casamino Acids was used as the positive control in these assays.