TABLE 2.
Summarized structures of OSEs derived from serotype 7 transformants
| GPL | Molecular weight of OSE | Fragment ions in MALDI-TOF/TOF MS |
O-Methyl group
|
Structure of OSE | |
|---|---|---|---|---|---|
| Terminal sugar | Residue next to terminal sugar | ||||
| GPL 7 vector | 859 | 254, 400, 546, 692 | 2-O-Met | 4N-acyl-4,6-dideoxy-2-O-Me-Hex→Rha→Rha→Rha→6-d-Tal | |
| GPL 7-orfA | 873 | 254, 414, 560, 706 | 2-O-Met | 4-O-Met | 4N-acyl-4,6-dideoxy-2-O-Me-Hex→4-O-Me-Rha→Rha→Rha→6-d-Tal |
| GPL 7-orfB | 859 | 254, 400, 546, 692 | 3-O-Met | 4N-acyl-4,6-dideoxy-3-O-Me-Hex→Rha→Rha→Rha→6-d-Tal | |
| GPL 7-orfAB | 873 | 254, 414, 560, 706 | 3-O-Met | 4-O-Met | 4N-acyl-4,6-dideoxy-3-O-Me-Hex→4-O-Me-Rha→Rha→Rha→6-d-Tal |