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Analysis of EPS size. (A) Western immunoblot analysis of whole-cell lysates demonstrating that the locked-on mutant (Δtsr19 M5) synthesizes a very-high-molecular-weight EPS not produced by the locked-off mutant (Δtsr19 M3) or the wild type. (B) Western immunoblot analysis of a whole-cell lysate of wild-type bacteria showing heterogeneous capsular polysaccharide H as a representative of the capsular polysaccharides. The sizes of molecular mass standards are indicated on the right.
