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. 2007 Nov 5;76(1):391–402. doi: 10.1128/IAI.01118-07

FIG. 1.

FIG. 1.

Southern blotting confirms the mutation in the BBA64 gene of lp54 in ML23 (noninfectious, lp25−, B. burgdorferi strain B31). (A and B) Total genomic DNA from the ML23 parent strain (lanes 1 and 2) and MM4 (BBA64 mt; lanes 3 and 4) was digested with EcoRV (lanes 1 and 3) and HinDIII (lanes 2 and 4) and probed with aadA (Strr marker; probe A) (A) or BBA64 (PCR amplified using BBA64F and BBA64R primers; probe B) (B). Numbers on the left of each panel indicate the sizes of the markers in kb. (C) Schematic of the BBA64 region of lp54 with the transposition site showing the customized transposon insertion (PflgB-Strr). The numbers indicate the gene designation within the BBA64 region of lp54. Hatched boxes indicate the respective locations of the Tn7 repeats. Arrows indicate the orientations of the BBA64R and BBA68F primers used to amplify the region of BBA64 on lp54. H, HinDIII; RV, EcoRV.