TABLE 1.
Plasmids and B. burgdorferi strains used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pCR2.1-TOPO | PCR cloning vector | Invitrogen |
| pBBE22 | Borrelial shuttle vector confers Kanr | 50 |
| pML102 | Customized donor plasmid for in vitro transposition | 61 |
| pMM2 | 4.5 kb of lp54 spanning BBA64 to BBA68 in pCR2.1-TOPO | This study |
| pMM4 | pMM2 with 1.6-kb insertion of PflgBaadA at 154 bp from 5′ end of BBA64 | This study |
| pBBE22/bba64+ | Borrelial shuttle vector with BBA64-BBA65 | This study |
| β-Actin/pCR2.1 | 347 bp of β-actin in pCR2.1-TOPO | This study; 47 |
| Strains | ||
| ML23 | B31, lp25−, noninfectious | 36 |
| MSK5 | B31 isolate with all infection-associated plasmids | 36 |
| MM4 | B31, lp25−, BBA64−, Strr | This study |
| ML23/pBBE22 (wt) | ML23 with pBBE22 (BBE22+), Kanr | This study |
| MM4/pBBE22 (mt) | MM4 with pBBE22 (BBA64− BBE22+), Strr Kanr | This study |
| MM4/pBBE22-bba64+ (ct) | MM4 with pBBE22/BBA64, (BBE22+ BBA64+), Strr Kanr | This study |