TABLE 2.
Oligonucleotides used in this study
| Primer designation | Sequence (5′→3′)a |
|---|---|
| BBA64F | ACGCCATATGGACAGCAATGAAAGCAAA |
| BBA64R | ACGCCTCGAGCTGAATTGGAGCAAGAAT |
| BBA65F | ACGCCATATGGATCTAAACAACAAAGAC |
| BBA68F | ACGCCATATGAGCAAAATCGATCCTAAA |
| Tn7N | ACTTTATTGTCATAGTTTAGATCTATTTTG |
| Tn7S | TCCCCACCTTTACCTCAAAAATTCCTAATA |
| actF | TCACCCACACTGTGCCCATC (Ref 47) |
| actR | GGATGCCACAGGATTCCAT (Ref 47) |
| flagellinF | TCTTTTCTCTGGTGAGGGAGC |
| flagellinR | CCAAGAAGGAAGGCTGGAAAA |
| LE/b-actinF | CAAGTCATCACTATTGGCAACGA |
| LE/b-actinR | CCAAGAAGGAAGGCTGGAAAA |
| E22F | ACGCGGTACCTTTTTATATTGTGAGCCGGTT |
| E22R | ACGCGGTACCTCTATGTATCCCCTTGTTCA |
a
Restriction sites underlined.