FIG. 3.

PCRan1 and PCMei2 complement meiotic defects in mutant fission yeast strains. A. S. pombe strain GP5286 h⁻ containing a meiotic defect related to Ran1 was transformed with either vector containing PCRan1 or vector alone and incubated at either the permissive 30°C or restrictive 37°C followed by DAPI staining to demonstrate either inhibition or progression of haploid meiosis. Expression of the vector insert was under control of the nmt (not made in thiamine) promoter. B. S. pombe strain FY13293 h⁹⁰ containing a meiotic defect related to Mei2 was transformed with PCMei2 or vector alone, incubated at 25°C, and stained with DAPI to observe the occurrence of premeiotic DNA synthesis, demonstrated as organisms containing four nuclear forms (encircled in white). In contrast, S. pombe strain FY13293 h⁹⁰ with vector alone did not exhibit any complementation.