FIG. 5.

PCRan1p exhibits protein kinase activity and is capable of phosphorylating PCMei2p. A. In vitro phosphorylation assay performed using the broad-spectrum serine-threonine kinase target PHAS-I, incubated either alone or in conjunction with purified PCRan1p at 30°C for 1 h in the presence of [γ-³²P]ATP. The products were separated on a 12% SDS-PAGE and visualized by autoradiography. B. In vitro phosphorylation assay performed as described above using either recombinantly expressed PCMei2p and heat-inactivated recombinantly expressed PCMei2p incubated either alone or in the presence of PCRan1p. The products were separated on a 7.5% SDS-PAGE and visualized as described above. PCRan1p was also incubated alone as a control for autophosphorylation.