FIG. 8.

Immunoelectron microscopy using specific antibodies to PCRan1p and PCMei2p antibodies shows localization of these meiotic control proteins over the life cycle of the organism. PCRan1p antibodies demonstrate localization of the protein as the life cycle progresses from trophic (A) to precyst (B) to mature cysts (C) within the intracystic bodies. PCRan1p is localized in trophic form and precyst cytoplasm but seems to selectively localize to the intracystic bodies in the mature cyst. In comparison, gold-labeled PCMei2p antibodies demonstrate protein localization in the trophic, precyst, and maturing cyst. Again, PCMei2p appears to localize predominantly to the intracystic bodies of maturing cysts.