TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| TUV 93-0 | EHEC O157:H7 strain EDL933, stx-negative (intimin γ) | ATCC |
| E2348/69 | Wild-type EPEC O127:H6 (intimin α) | 25 |
| ICC223 | E. coli O125:H6 isolated in Brazil from a diarrheagenic case (intimin α) | This study |
| CPG35 | E. coli O125:H6 isolated in the United Kingdom from a diarrheagenic case (intimin α) | This study |
| N67 | E. coli O125:H6 isolated in the United Kingdom from a diarrheagenic case (intimin α) | This study |
| 2741-5 | E. coli O125:H6 isolated in Brazil from a diarrheagenic case (intimin α) | This study |
| ICC224 | ICC223Δtir::Km | This study |
| ICC225 | Δtir in EPEC O127:H6 strain E2398/69 | This study |
| TUV 93-0Δtir | Δtir in EHEC O157:H7 | 22 |
| Plasmids | ||
| pACYC-P | pACYC derivative encoding mtc (map tir cesT 5′ eae) from E2348/69 | 18 |
| pKD46 | Helper plasmid encoding λ red recombinase | 8 |
| pKD4 | Template | 10 |
| pSA10 | pKK177-3 derivative containing lacQ | 31 |
| pICC368 | pSA10 derivative encoding TirICC223 | This study |
| pICC369 | pSA10 derivative encoding TUV 93-0 TccP-HA fusion protein | This study |