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The ΔOx mutant has the transposon inserted in the MAP3464 gene. (A) PCR from genomic DNA using specific primers for the MAP3464 and 16S RNA genes. Lanes: MW, molecular weight marker; 1, ΔOx mutant; 2 and 3, wt M. avium subsp. paratuberculosis; 4, PCR negative control. (B) Organization of the oxidoreductase operon in M. tuberculosis and M. avium subsp. paratuberculosis genomes.
