FIG. 4.

Differential accumulation of IL-12-related subunit mRNAs in cDC subsets. CD11c^hi DC subsets were sorted on the basis of CD4 and CD8α expression from naïve and infected (2 × 10⁷ [LD] or 2 × 10⁸ [HD] parasites/mouse). (A) IL-12/23p40 mRNA, (B) IL-23p19 mRNA, and (C) IL-27p28 mRNA accumulation in CD4⁺, CD8α⁺, and DN DC were examined by real-time RT-PCR. Target genes were normalized against HPRT. Data represent the means plus standard errors of the means (error bars) for three separate experiments.