FIG. 5.
Regulation of Th cell differentiation by IL-12/23p40 and Delta 4 Notch-like ligand. (A) Sorted DC subsets (1 × 104) from naïve C57BL/6 mice and C57BL/6 mice infected with 2 × 107 (LD) or 2 × 108 (HD) amastigotes were cultured with purified naïve OT-II T cells (1 × 105/well) in the presence of 0.1 μg/ml OVA peptide and in the presence of either a control rat immunoglobulin G2a (IgG2a) MAb (left panels) or anti-IL-12/23p40 MAb (right panels). One week later, the cells were restimulated with whole spleen cells from B6.CD45.1 mice and 2 μg/ml OVA peptide for 7 h and subsequently stained for IFN-γ and IL-4. Plots were gated on CD4+ CD45.1− cells. The numbers indicate the percentage of events within the two quadrants. (B and C) IL-12/23p40 (B) and Delta 4 Notch-like ligand (C) mRNA accumulation for each cDC subset from infected mice is shown relative to mRNA accumulation in the same subset isolated from naïve mice. Data are derived from one experiment using cells obtained from five mice as cDC donors.