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In vitro transformation experiments. By their abilities to convert to metacyclic trypomastigotes, we determined the transition efficiencies of WT and L16 clone epimastigotes by adding 1% triatomine gut homogenate to the cultures. The L16 clone showed a significant increase in the percentage of trypomastigotes compared to that of the WT (P < 0.05). The results plotted are the means ± standard errors of the means (error bars) of two different experiments.
