FIG. 5.

Analysis of frequencies of CD4⁺ T cells producing IL-2, IFN-γ, and IL-2 plus IFN-γ by ICS. PBMC from rhesus monkeys receiving LSA1/AS01B (A), RTS,S/AS01B (B), or LSA1/AS01B and RTS,S/AS01B simultaneously (C) were collected 2 weeks after the last immunization and then were stimulated with different pooled peptides in a short-term restimulation in vitro assay. Cytokine-producing CD4⁺ CD8⁻ Τ cells were separated into three distinct populations based on the production of IL-2 (black bars), IFN-γ (hatched bars), and IL-2 plus IFN-γ (white bars). Data shown are means ± SE.