FIG. 2.
IL-8 induction in epithelial cells challenged with the BspA protein. HGECs were treated with rBspA (5 μg/ml) or the TLR2 agonist Pam3Cys (Pam3) (0.1 μg/ml) for 6 h. Induction of IL-8 in culture supernatants was determined by an ELISA. The data are the means ± standard deviations of triplicate determinations in one of three independent sets of experiments that yielded similar findings. Statistically significant (P < 0.05) blocking of cytokine release by TLR2 antibody (Ab) is indicated by an asterisk.