TABLE 2.
MOI | IL-8 secretion (pg/ml) in response toa:
|
|
---|---|---|
Parent strain | BspA mutant | |
20 | 1,680 ± 178 | 1,038 ± 110 |
200 | 2,897 ± 326 | 2,734 ± 301 |
Monolayers of stable HEK293 (hTLR2/hTLR1) cells were stimulated with T. forsythia parent strain ATCC 43037 or BspA mutant strain BFM571 at different MOIs. Culture supernatants were collected after 12 h and assayed for IL-8 by an ELISA. There was a significant difference (P < 0.05) in the IL-8 response between the parent and mutant strains at an MOI of 20, but there was not a significant difference at an MOI of 200.