FIG. 5.

(A) Biophotonic image of a chinchilla from the cohort that received rabbit anti-human CEA antibody 20 min prior to challenge with a luminescent NTHI reporter strain. This image was captured 19 h after bacterial challenge and shows no detection of luminescence from the nasal cavity (yellow boxed region). A similar image from an animal that received naïve rabbit serum is shown in panel B and demonstrates the detection of luminescence from the nasal cavity of this chinchilla (see the yellow boxed region). (C) Biophotonic image of NP lavage fluids recovered 24 h after bacterial challenge from animals in the cohorts that received either rabbit anti-human CEA (left tube) or naïve rabbit Ig (right tube). (D) Relative clearance of NTHI from the NP of chinchillas in cohorts that received either naïve rabbit serum (red bars) or rabbit anti-human CEA (blue bars) prior to intranasal bacterial challenge. Chinchillas that received anti-human CEA antibody cleared NTHI from their NP significantly earlier than did those that received naïve rabbit serum (P = 0.031). (E) Biophotonic image of NP lavage fluids recovered 24 h after bacterial challenge from animals that received either rabbit anti-human CEA antiserum (tube on left) or rabbit anti-human E-cadherin antiserum (tube on right).