FIG. 6.

MYD88 is required for E. histolytica DNA-induced TNF-α expression. Bone marrow-derived macrophages were propagated from wild-type and MYD88^−/− mice and stimulated in vitro for 3 h with media, LPS (1 μg/ml), or E. histolytica (E.h) DNA (30 μg/ml). TNF-α mRNA expression was measured by real-time PCR (A), and PCR products were processed on 1.5% agarose gels (B). Data points represent means ± standard errors of the means of the results obtained from three experiments. An asterisk indicates a significant decrease in TNF-α expression compared to wild-type macrophage results (P < 0.05).