TABLE 4.
Comparison of qRT-PCR and microarray dataa
| ORF | Fold up- or down-regulation
|
|
|---|---|---|
| Microarray | qRT-PCRb | |
| S1 antigen BBA05 | 3.39 | 1.2 |
| BBA64 | 10.97 | 2.46 |
| BBA71 | 25.1 | 1.48 |
| BBA72 | 11.6 | 3.5 |
| BBA73 | 10.2 | 1.8 |
| BB0337 | −2.5 | 3.4 |
| cheX BB0671 | 2.51 | 9.8 |
| cheW BB0670 | 5.14 | 22.6 |
| BBA52 | −2.63 | 1.5 |
| OspC BBB19 | 9.82 | −1.43 |
| ospA BBA15 | 3.41 | 8.9* |
| dbpA BBA24 | 2.60 | 21* |
| dbpB BBA25 | 2.01 | 19* |
| BBK32 | 1.35 | −3.33* |
a
The agreement between the two methods was 70%.
b
ORFs marked with an asterisk did not pass all three statistical tests in the microarray analysis.