FIG. 4.

Transmission electron micrographs showing glycogen staining of high-pressure frozen, freeze-substituted, and Epon-embedded thin sections of four anammox genera. All investigated anammox genera show glycogen staining in the riboplasmic compartment. (A and B) “Candidatus Kuenenia stuttgartiensis.” (B) Negative control incubated with water instead of periodic acid. (C) “Candidatus Anammoxoglobus propionicus.” (D) “Candidatus Brocadia fulgida.” (E and F) “Candidatus Scalindua spp.” cells with (E) and without (F) pilus-like appendages. Scale bars, 200 nm.