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Interaction of LmrR with the lmrCD and lmrR promoter regions. EMSA was performed with increasing amounts of purified LmrR incubated with 0.7 μM of 32P-labeled 369-bp and 387-bp double-stranded DNA (dsDNA) probes comprising the promoter regions of lmrCD (A) and lmrR (B), respectively. Where indicated, wild-type LmrR was substituted for the LmrR(T82I) mutant. *, single-stranded probe DNA. Unless indicated otherwise, LmrR was used at a concentration of 3.7 μM.
