TABLE 1.
Primers used
| Primer | Sequence (5′ to 3′)a | Endonuclease |
|---|---|---|
| lmrR FW1 | GGCCCATGGGGGCAGAAATACCAAAAGAAATG | NcoI |
| lmrR RV1 | GCGTCTAGATTTAATCGCTTTCACTCTTCTTAT | XbaI |
| lmrR FW2 | TATAGATCTGCAATTCGAAGTCCAATTAAG | BglII |
| lmrR RV2 | TATGGATCCGTAAGTTGCTTCACGAACGTC | BamHI |
| lmrR FW3 | TAAATTCACGATTCATTCCTTACTT | |
| lmrR RV3 | TCTTTTTCCTTTCTATCATTTTAAACA | |
| lmrCDpmtr FW1 | ATTGTAATCTTTAACAGCATTAAC | |
| lmrCDpmtr FW2 | ACAAATAACGTCGTAAATCG | |
| lmrCDpmtr RV1 | GGCAACCCATTTATGCTTCA | |
| lmrRpmtr FW1 | TGTCGCAAACGCAATTTGTC | |
| lmrRpmtr FW2 | TCAAGGAAAGTTGTCTTCCACCGCTAA | |
| lmrRpmtr RV1 | CTGCCATTCTTTTTCCTTTC | |
| lmrRpmtr RV2 | GGGCTCGTAACATTTCTTTTGGTATTTCTG | |
| lmrC RT-PCR FW | GTTGAAGAACGTGGGAATAATTTCTCAGGTGG | |
| lmrC RT-PCR RV | CCTCCTGTGCTTTCTGTGTATCGTAGATTTC | |
| lmrD RT-PCR FW | CGTTTCTGATGATGAATCAGTCTTCTCAGTTGG | |
| lmrD RT-PCR RV | CAAAAACGAATTGATTATGATAAAGTTCAGAG | |
| lmrR RT-PCR FW | ATGGCAGAAATACCAAAAGAAATG | |
| lmrR RT-PCR RV | TTATTTAATCGCTTCACTCTTCTTAT | |
| secY RT-PCR FW | TACAACTGCTCCAGCTACGA | |
| secY RT-PCR RV | GTTCCTCCAAGAGCGACAAT |
a
Endonuclease sites are in bold.