FIG. 1.
Analysis of sigma factor protein similarity and real-time RT-PCR analysis of sigma factor gene expression in the M. tuberculosis sigB and sigF KI recombinant strains. (A) A phylogenetic tree of the 13 M. tuberculosis sigma factors was generated by comparison of the amino acid sequences of these sigma factors and the sequence of Vibrio RpoS (obtained from NCBI GenBank accession no. AAK09363). (B) Changes in gene expression of the 13 sigma factors in the M. tuberculosis sigB KI recombinant strain were measured 6 and 12 h after acetamide induction by using real-time RT-PCR. The CT value was normalized to that of sigA, and the relative expression was calculated by the ΔΔCT method compared to the control strain. Bars: □, 6 h; ░⃞,12 h. (C) Same analysis as in panel B but with the M. tuberculosis sigF KI recombinant strain.