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. 2007 Nov 9;190(2):699–707. doi: 10.1128/JB.01273-07

FIG. 3.

FIG. 3.

In vitro transcription assays using purified SigB and SigF sigma factors. (A) In vitro transcription assay using sigma factor protein and DNA template involving the 5′ UTRs of the sigB, ideR, and sigC genes. Lane M, RNA size markers (Ambion). All lanes correspond to complete in vitro transcription reaction mixes with the presence or absence of sigma factor and the presence of either long (template 2) or short (template 1) promoter-containing target templates. Lanes: 1, no added SigB/+sigB long template; 2, SigB added/+sigB long template; 3, SigB added/+sigB short template; 4, no added SigB/+ideR long template; 5, SigB added/+ideR long template; 6, SigB added/ideR short template; 7, no SigF added/+sigC long template; 8, SigF added/+sigC long template; 9, SigF added/+sigC short template. (B) Diagram of expected lengths of transcript products from the short and long templates. The transcription start site of sigB was previously identified (19).