TABLE 4.
Orphan histidine protein kinase genes in M. xanthus transcriptionally up-regulated during development
| TIGR_MXAN (gene symbol) | Max. expression ratio (microarrays)/induction time (h)a,b | Max. expression ratio (qRT-PCR)/induction time (h)a,b | No. of TM helicesc | Domain organizationd |
|---|---|---|---|---|
| MXAN0060 | NOA | 19.3×-up/0-6 | 2 | HisKA-HATPase_c |
| MXAN0176 | NOA | 14.8×-up/6-12 | 0 | HisKA-HATPase_c |
| MXAN0340 | 2.7×-up/4-6 | ND | 0 | HisKA-HATPase_c |
| MXAN0571 | 1.9×-up/2-4 | ND | 0 | HisKA-HATPase_c |
| MXAN0706 | NOA | 3.4×-up/0-6 | 0 | HisKA-HATPase_c |
| MXAN0712 | 1.6×-up/12-15 | ND | 0 | HisKA-HATPase_c-RR-RR |
| MXAN0736 | 2.2×-up/0-2 | 128×-up/0-6 | 2 | HisKA-HATPase_c |
| MXAN0928 | 10.9×-up/2-4 | 29.7×-up/0-6 | 0 | HisKA-HATPase_c |
| MXAN0931 (= espA) | 4.4×-up/0-2 | ND | 0 | HisKA-HATPase_c-RR |
| MXAN1014 (= sdeK) | 2.6×-up/0-2 | ND | 0 | HisKA-HATPase_c |
| MXAN2763 | 2.4×-up/0-2 | ND | 0 | HisKA-HATPase_c-RR |
| MXAN3036 | 2.1×-up/4-6 | 29.8×-up/0-6 | 3 | HisKA-HATPase_c |
| MXAN3098 | 1.9×-up/0-2 | 4.1×-up/0-6 | 0 | HisKA-HATPase_c |
| MXAN3290 | 2.8×-up/0-2 | 4.4×-up/0-6 | 0 | HisKA-HATPase_c-RR |
| MXAN4465 | 2.1×-up/4-6 | ND | 0 | HisKA-HATPase_c-RR |
| MXAN4988 | 1.6×-up/0-2 | 2.8×-up/0-6 | 8 | HisKA-HATPase_c |
| MXAN5483 | 2.2×-up/4-6 | ND | 0 | HisKA-HATPase_c |
| MXAN6315 | 4.2×-up/0-2 | 36.5×-up/0-6 | 0 | HisKA-HATPase_c-RR |
| MXAN6855 (espC) | 1.7×-up/12-15 | ND | 8 | HisKA-HATPase_c-RR |
| MXAN6941 | NOA | 7.5×-up/6-12 | 0 | HisKA-HATPase_c |
| MXAN6994 | 4.4×-up/2-4 | 76.4×-up/0-6 | 0 | HisKA-HATPase_c |
| MXAN6996 (asgD) | 1.8×-up/12-15 | ND | 0 | RR-HisKA-HATPase_c |
| MXAN7123 | 4.0×-up/2-4 | 143×-up/0-6 | 0 | HisKA-HATPase_c |
| MXAN7206 (mokA) | NOA | 9.2×-up/0-6 | 0 | HisKA-HATPase_c-RR |
| MXAN7398 | NOA | 3.8×-up/0-6 | 0 | HisKA-HATPase_c |
Expression ratios were calculated as the expression in developing cells over the expression in vegetative cells. Maximum (max.) expression ratios indicate the maximum expression ratio for a particular gene during all time points tested using DNA microarrays (0, 2, 4, 6, 9, 12, 15, 18, and 24 h) and qRT-PCR (0, 6, 12, 18, and 24 h). Induction time indicates the time interval in which the expression ratio began to change relative to that observed in vegetative cells. “-up” indicates up-regulation.
NOA, gene not represented or not detected on DNA microarray; ND, not determined.
TM, transmembrane.
All domains present in a protein are indicated except for domains in the sensor domain.