TABLE 1.
Bacterial strains and plasmids used in this study
| Strain(s) or plasmid | Description | Reference or source |
|---|---|---|
| K. pneumoniae strains | ||
| Clinical isolates | 74 isolates collected from National Taiwan University Hospital from 1997 to 2003 | 18 |
| NTUH-K2044 | Clinical strain causing PLA and meningitis | 18 |
| MGH78578 | ATCC 700721, used for determination of the complete genome sequence at Washington University | ATCC |
| NTUH-K2044 int mutant | NTUH-K2044 isogenic int mutant (insertion mutation) | This study |
| NTUH-K2044 irp2 mutant | NTUH-K2044 isogenic irp2 mutant (irp2 replaced by a kanamycin resistance gene) | This study |
| NTUH-K2044 rmpA mutant | NTUH-K2044 isogenic rmpA mutant (mutant 3-20, transposon insertion mutant) | 18 |
| NTUH-K2044 virB1 mutant | NTUH-K2044 isogenic virB1 mutant (insertion mutation) | This study |
| NTUH-K2044 mobB mutant | NTUH-K2044 isogenic mobB mutant (insertion mutation) | This study |
| N4252 Rifr | Non-tissue-invasive strain, spontaneous rifampin-resistant mutant | This study |
| E. coli strains | ||
| HB101 | supE44 hsdS20(rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 | Takara |
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen | 23 |
| S17-1λpir | hsdR recA pro RP4-2 (Tc::Mu; Km::Tn7)(λpir) | 31 |
| Plasmids | ||
| pACYC184 | Cloning vector, P15A replicon, Cmr | New England Biolabs |
| pUT-km | pUTKm1-derived plasmid with mini-Tn5 excised by EcoRI, tnp excised by SalI, and bla removed by ApaLI and with insertion of a kanamycin resistance cassette from pUC4K into a PstI site | 17 |