TABLE 3.

ORFs in the middle and 3′ regions of ICEKp1

ORF		Product size (amino acids)	Locationa		Homologous protein(s)	Source	Amino acid identityb		Accession no.
No.	Designation		Start site	Stop site			No. of matching amino acids	Total no. of amino acids
1	ORF1	203	36256	35645	Transposase and inactivated derivatives	Y. pestis	179	188	ZP_00797222
2	ORF2	63	36371	36180	IS100 ORF1	Shigella dysenteriae	60	63	ZP_406030
3	ORF3	300	37368	36466	Transposase and inactivated derivatives	Y. pestis	297	300	ZP_01175356.1
4	ORF4	126	37936	37556	Hypothetical protein
5	ORF5	76	38379	38609	VagC	Salmonella enterica	71	75	ZP_271744.1
6	ORF6	138	38606	39022	VagD	K. pneumoniae CG43	135	138	NP_943352.1
7	ORF7	87	39040	39303	Hypothetical protein LV173	K. pneumoniae CG43	51	74	NP_943463.1
8	iroN	724	41632	39458	IroN	E. coli UTI89	686	725	ZP_540133.1
9	iroB	371	42504	43619	IroB	K. pneumoniae CG43	362	371	NP_943394.1
10	iroC	1231	43706	47401	IroC	K. pneumoniae CG43	1198	1214	NP_943393.1
11	iroD	409	47507	48736	IroD	K. pneumoniae CG43	404	409	NP_943392.1
12	pagO	304	50655	49741	PagO	K. pneumoniae CG43	275	300	NP_943390.1
13	ORF13	183	51279	50728	Hypothetical protein
14	rmpA	210	52286	51654	Regulator of mucoid phenotype	K. pneumoniae CG43	183	210	AAL25252.1
15	ORF15	54	52300	52464	Hypothetical protein
16	ORF16	217	52828	53481	Hypothetical protein LV256	K. pneumoniae CG43	175	179	NP_943388.1
17	ORF17	61	54304	54119	Transposase and inactivated derivatives	Y. pestis	61	61	ZP_00797222.1
18	ORF18	77	54789	55022	Putative ATP/GTP-binding protein remnant	Y. pestis	46	66	NP_995420
19	virB1	236	55095	55805	Pilx1/VirB1-like protein	E. coli ECOR31	232	236	AAP70283.1
20	virB2	97	55805	56098	VirB2-like protein	E. coli ECOR31	97	97	AAP70284.1
21	virB3-virB 4	912	56111	58849	Pilx3-4/VirB3-4-like protein	E. coli ECOR31	897	912	AAP70302.1
22	virB5	234	58867	59571	Pilx5/VirB5-like protein	E. coli ECOR31	224	229	AAP70285.1
23	virB6	359	59820	60899	Pilx6/VirB6-like protein	E. coli ECOR31	296	354	AAP70286.1
24	virB8	227	61115	61798	Type IV secretion system VirB8 component	Y. pestis	226	227	NP_995427.1
25	virB9	302	61795	62703	Type IV secretory pathway VirB9 component	Y. pestis	293	301	NP_995428.1
26	virB10	416	62747	63997	Pilx10/VirB10-like protein	E. coli ECOR31	402	416	AAP70288.1
27	virB11	341	63987	65012	Type IV secretory pathway VirB11 component	Y. pestis	335	341	NP_995430.1
28	ORF28	132	65009	65407	Hypothetical protein YP_pCRY17	Yersinia pestis	118	132	NP_995431.1
29	ORF29	100	65443	65745	YggA-like protein	E. coli ECOR31	69	101	AAP70290.1
30	ORF30	101	65788	66093	Unknown	E. coli ECOR31	83	101	AAP70307.1
31	ORF31	100	66204	66506	Unknown	E. coli ECOR31	99	100	AAP70308.1
32	mobB	629	66773	68662	MobB-like protein	E. coli ECOR31	607	629	AAP70291.1
33	mobC	248	68672	69418	MobC-like protein	E. coli ECOR31	212	245	AAP70292.1
34	ardC	315	70561	69614	Antirestriction protein	E. coli ECOR31	290	315	AAP70295.1
35	ORF35	271	72361	71546	Hypothetical protein
36	ORF36	115	73009	72662	Hypothetical protein Nham_1368	N. hamburgensis	50	93	ZP_576652.1
37	ORF37	464	74403	73009	UBA/THIF-type NAD/flavin adenine dinucleotide binding fold	N. hamburgensis	235	459	ZP_576653.1
38	ORF38	375	75503	74376	Hypothetical protein Nham_1370	N. hamburgensis	176	353	ZP_576654.1
39	ORF39	182	76146	75598	Conserved hypothetical protein	Gram-negative bacterium	93	175	AAQ10297.1
40	ORF40	367	77292	76189	Hypothetical protein Shewmr7DRAFT_1704	Shewanella sp. strain MR-7	147	365	ZP_00855252.1

^aNucleotide position in the GenBank accession no. AB298504 sequences.

^bDetermined by BLAST-P analysis.