TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Antibiotic resistance | Reference |
|---|---|---|
| E. coli | ||
| BL21(DE3) | Stratagene | |
| DH5α | Stratagene | |
| S. pneumoniae | ||
| D39 wild type | NCTC 7466 | |
| D39ΔcodY | Trimethoprim | This study |
| D39Δcps | Kanamycin | 7 |
| D39ΔcpsΔcodY | Kanamycin, trimethoprim | This study |
| D39ΔcpsΔpcpA | Erythromycin, kanamycin | This study |
| D39ΔcpsΔcodYΔpcpA | Kanamycin, trimethoprim, erythromycin | This study |
| Plasmids | ||
| pBlueScript KS+ | Stratagene | |
| pCR2.1 | Invitrogen | |
| pET11C | New England BioLabs | |
| pR412T7 | 4 | |
| pKOT | This study | |
| pKOCOD | This study |