TABLE 2.
Primers used for PCR amplification
| Primer | Primer sequencea | Purpose |
|---|---|---|
| CAT3 | GGTTATACTAAAAGTCGTTTGTTGGTTC | Primer extension |
| DTrev | GCGAGCTCAACTTGTATGTGGTAACTTATGC | R5+R6 deletions |
| lac5pX | GCTCTAGACTGCATTGTTAAAATATGTATC | EMSA |
| lac3pSc | GCGAGCTCGTTTAAATTATAAACATAAAC | EMSA |
| Protbio | 5Bio-tctagaCAGTAGATGCTCATCTTTTTTTAG | Affinity purification |
| R13pSc | GCGAGCTCGAAAATATATCAATATTACCATTAAATTG | R1 deletion |
| R23pSc | GCGAGCTCATTTTATAATTTATATACAAAG | R2 deletion |
| R33pSc | GCGAGCTCAGAAATAAGATAATAGTAC | R3 deletion |
| R43pSc | GCGAGCTCGTTGAATAAAATTAAATAAG | R4 deletion |
| R55pX | GCTCTAGATATTCAACTTTGACAATTGAATAG | R5 deletion |
| R65pX | GCTCTAGACTTATTTCTAAATATTAACTC | R6 deletion |
| rot3pSc | GAGCTCGTGTAAATAAACTTGCTTTC | EMSA |
| rot5pX | CGGGATCCTTTGTTAATACTTGTATAG | EMSA |
| rot5p2X | TCTAGACAGTAGATGCTCATCTTTTTTTAG | R1-R4 deletions |
| RotHis3pS | GTCGACTTAGTGATGGTGATGGTGATGCACAGCAATAATTGCGTTTAAAC | Construction of pHH3735 |
| RotHis5pX | TCTAGACAGTAGATGCTCATCTTTTTTTAGAACTTT | Construction of pHH3735 |
| Rotprom3p | GCTAAACATCTCCCAATTAATAATC | Affinity purification |
a
Underlining signifies bases added to primers to create restriction endonuclease recognition sites.