FIG. 5.

Involvement of rhlI in low-iron-stimulated twitching motility. P. aeruginosa strains K2589 (RhlI⁺), K2590 (RhlI⁻), K2590 harboring rhlI on low-copy-number plasmid pGP003, and K2590 with pMMB207 alone were inoculated onto iron-limited BM2 glucose (a), iron-replete (100 μM FeCl₃) BM2 glucose (b), and L agar (c) twitching plates and twitching zones (diameter) measured after 24 (L agar) or 40 (minimal media) h of incubation at 37°C. Error bars represent the standard errors of the means for a representative assay performed at least in triplicate.