TABLE 1.
Bacterial strains and plasmids
| Species and strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli | ||
| Sm10(λpir) | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu; Kmr λpir | 48 |
| DH5α | φ80d lacZΔM15 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 F− Δ(lacZYA-argF)U169 | 5 |
| S17-1 | thi pro hsdR recA Tra+ | 74 |
| P. aeruginosa | ||
| PAO1 | Prototroph | N. Gotoh, Kyoto Pharmaceutical University |
| K1120 | PAO1 ΔaphA; Kms | N. Gotoh, Kyoto Pharmaceutical University |
| K1203 | K1120 ΔpvdD | 72 |
| K2873 | K1120 ΔrhlR | This study |
| K2589 | K1120::PA4570-lacZ; carries a chromosomal insertion of a PA4570-lacZ transcriptional fusion | This study |
| K2590 | K2589 rhlI::mini-Tn5-tet; Tcr | This study |
| K2592 | K2589 mexI::mini-Tn5-tet; Tcr | This study |
| PAO1 | Prototroph | 44 |
| PAO1-rhlI | PAO1 rhlI::mini-Tn5-luxCDABE; Tcr | 44 |
| MPAO1 | Prototroph | 33 |
| MPAO1-rhlI | PAO1 rhlI::ISphoA/hah; Tcr | 33 |
| Plasmids | ||
| pUC18 | Cloning vector; Apr | 83 |
| Mini-CTX-lacZ | Integration vector with promoterless lacZ and oriT; Tcr | 9 |
| pGP001 | Mini-CTX::PA4570-lacZ; PA4570 promoter region cloned upstream of the promoterless lacZ gene of mini-CTX-lacZ | This study |
| pFLP2 | Source of Flp recombinase; Apr | 28 |
| pK18mobsacB | Broad-host-range gene replacement vector; sacB Kmr | 74 |
| pMMB207 | Broad-host-range low-copy-no. cloning vector; Cmr | 49 |
| pGP003 | pMMB207::rhlI | This study |
| pEX18Tc | Broad-host-range gene replacement vector; sacB Tcr | 28 |
| pCG001 | pEX18Tc::ΔrhlR | This study |
| pECP61.5 | pSW205::rhlA-lacZ; Apr | 58 |
| pMRP-1 | pUCP18::gfp; Apr Cbr | 17 |
a
Tcr, tetracycline resistant; Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kms, kanamycin sensitive; Kmr, kanamycin resistant; Cbr, carbenicillin resistant.