TABLE 1.
Bacterial strains and plasmids used in this study
| Strains or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| C58 | Wild-type pathogenic strain of A. tumefaciens | ATCC 33970 |
| A002 | nir strain of A. tumefaciens with Campbell insertion of pSUP202 into nirK ORF; Tcr | 3 |
| A011 | nnrR strain of A. tumefaciens with nnrR being replaced by ΩSprSmr cassette; Smr Spr | 3 |
| BER3 | fnrN strain of A. tumefaciens: fnrN::pBER213; Cbr, Sucs | 28 |
| RG8 | sinR strain of A. tumefaciens: sinR::TnMod-OKm′; Kmr | 28 |
| A059 | actR strain of A. tumefaciens with Campbell insertion of pJP5603 into actR ORF; Kmr | This study |
| A071 | fixN strain of A. tumefaciens with Campbell insertion of pSUP202 into fixN ORF; Tcr | This study |
| A105 | fixK strain of A. tumefaciens with Campbell insertion of pSUP202 into fixK ORF; Tcr | This study |
| A111 | paz strain of A. tumefaciens with Campbell insertion of pJP5603 into paz ORF; Kmr | This study |
| Plasmids | ||
| pWL-CYCA | Derivative of pYSW35 with PrrnB′-cycA fusion | 18 |
| pBBRAnirK | Derivative of pBBR1MCS-5 (18) with 426 bp upstream of nirK ORF (Gmr) | This study |
| pETactR | Derivative of pET-16b with ORF of actR | This study |
| pACTR-I | Derivative of pJP5603 containing an internal fragment of actR (35 bp-347 bp); Kmr | This study |
| pPAZ-I | Derivative of pJP5603 containing an internal fragment of paz (81 bp-313 bp); Kmr | This study |
| pFIXK-I | Derivative of pSUP201 containing an internal fragment of fixK (56 bp-594 bp); Tcr | This study |
| pFIXN-I | Derivative of pSUP201 containing an internal fragment of fixN (669 bp-1101 bp); Kmr | This study |
| pAnorCZ | Derivative of pRK415 with norB-lacZ transcriptional fusion (Tcr Kmr) | 3 |
| pAnirKZ | Derivative of pRK415 with nirK-lacZ transcriptional fusion (Tcr Kmr) | 3 |
| pAnnrRZ | Derivative of pRK415 with nnrR-lacZ transcriptional fusion (Tcr Kmr) | This study |
| pApazZ | Derivative of pRK415 with paz-lacZ transcriptional fusion (Tcr Kmr) | This study |
| pAnirKZ | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 208 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK2Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 149 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK3Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 78 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK4Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 127 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK5Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 110 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK6Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 97 bp upstream of the nirK start (Tcr Kmr) | This study |
| pAnirK7Z | Derivative of pRK415 with nirK-lacZ transcriptional fusion containing 92 bp upstream of the nirK start (Tcr Kmr) | This study |
| pnirM4 | Derivative of pRK415 with a mutation in the ActR binding site of the nirK promoter (G→C, C→A, and C→T; Tcr) | This study |
| pnirM5 | Derivative of pRK415 with a mutation in the ActR binding site of the nirK promoter (C→A and C→T; Tcr) | This study |
| pnirM6 | Derivative of pRK415 with a mutation in the ActR binding site of the nirK promoter (G→C; Tcr) | This study |
| pnirM4Z | Derivative of pNIRM4 with nirKM4-lacZ transcriptional fusion (Tcr Kmr) | This study |
| ppazM1 | Derivative of pRK415 with a mutation in the ActR binding site of the paz promoter (G→T, G→T, and C→T; Tcr) | This study |