TABLE 2.
Expression of nirK-lacZ, norC-lacZ, and nnrR- lacZ in A. tumefaciens strains
| Strain (genotype) | β-Galactosidase activity (Miller units) under the indicated conditionsa
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
|
nirK-lacZ
|
norC-lacZ
|
nnrR-lacZ
|
|||||||
| Aerobic | O2 limited | O2 limited + NO3− | Aerobic | O2 limited | O2 limited + NO3− | Aerobic | O2 limited | O2 limited + NO3− | |
| C58 | 5 ± 2 | 805 ± 25 | 1307 ± 51 | 3 ± 2 | 591 ± 21 | 902 ± 27 | 130 ± 11 | 637 ± 33 | 753 ± 28 |
| BER3 (fnrN) | 3 ± 2 | 48 ± 8 | 71 ± 9 | 8 ± 3 | 80 ± 5 | 67 ± 9 | 77 ± 4 | 88 ± 6 | 115 ± 7 |
| RG8 (sinR) | 6 ± 4 | 433 ± 29 | 1063 ± 56 | ND | ND | ND | 115 ± 7 | 281 ± 13 | 386 ± 19 |
| A011 (nnrR) | 4 ± 2 | 137 ± 10 | 110 ± 9 | 1 ± 1 | 33 ± 5 | 28 ± 4 | 136 ± 9 | 450 ± 12 | 411 ± 27 |
| A059 (actR) | 4 ± 3 | 17 ± 8 | 129 ± 13 | 6 ± 2 | 52 ± 5 | 278 ± 12 | 124 ± 14 | 411 ± 24 | 697 ± 25 |
| A071 (fixN) | 5 ± 3 | 736 ± 31 | 1288 ± 43 | ND | ND | ND | 131 ± 8 | 514 ± 18 | 426 ± 16 |
| A105 (fixK) | 6 ± 2 | 607 ± 38 | 1206 ± 30 | ND | ND | ND | 129 ± 6 | 506 ± 34 | 603 ± 30 |
a
Activities ± standard deviations were determined in duplicate assays of at least three independently grown cultures. ND, not determined.