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Transcriptional analysis of the as-48ABC genes in JH2-2[pAM401-81] and JH2-2[pAM401-81X] strains. (A) Northern blotting detection using specific probes derived from the as-48A gene. RNA was extracted at the indicated OD600. O.D., optical density. (B) Semiquantitative RT-PCR analysis of as-48A (upper panel), as-48AB (middle panel), and as-48BC (lower panel) mRNAs in the indicated strains. The plasmid carried by the JH2-2 strain and the number of cycles of each sample are indicated.
