TABLE 2.
Sugar composition of cell walls from members of the B. cereus group
| MLST clade/lineage | Strain | Sugar composition (% ± SD)a
|
|||||
|---|---|---|---|---|---|---|---|
| Man | Glc | Gal | ManNAc | GlcNAc | GalNAc | ||
| 1/Anthracis | B. anthracis Ames | ND | 6.2 ± 1.1 | 54.2 ± 7.4 | 13.2 ± 4.3 | 26.2 ± 4.2 | ND |
| B. anthracis Pasteur 4229 | ND | 5.6 ± 1.2 | 52.3 ± 7.7 | 13.2 ± 3.6 | 28.6 ± 4.9 | ND | |
| B. anthracis Sterne 34F2 | ND | 8.5 ± 1.7 | 61.3 ± 6.7 | 4.7 ± 2.1 | 25.4 ± 5.0 | ND | |
| B. anthracis 7702 | ND | 15.0 ± 1.6 | 54.5 ± 8.6 | 8.1 ± 5.8 | 21.3 ± 2.4 | ND | |
| B. anthracis UT60b | ND | 23.0 ± 1.1 | 49.3 ± 3.1 | 7.2 ± 0.9 | 20.2 ± 3.0 | ND | |
| 1/Cereus I | B. cereus F666 | ND | 24.5 ± 7.9 | 13.2 ± 5.8 | 12.8 ± 3.6 | 32.3 ± 6.5 | 16.8 ± 3.7 |
| B. cereus ATCC 10987 | ND | 2.6 ± 1.3 | 31.8 ± 6.2 | 15.4 ± 0.2 | 25.7 ± 3.0 | 24.5 ± 4.2 | |
| 1/Cereus III | B. cereus B5780 | 2.7 ± 1.4 | 68.3 ± 2.7 | 1.2 ± 0.2 | 2.9 ± 1.5 | 24.9 ± 3.2 | ND |
| B. cereus 03BB102 | 0.9 ± 0.5 | 5.1 ± 0.8 | 61.7 ± 5.8 | 9.5 ± 3.9 | 22.9 ± 2.7 | ND | |
| 1/Cereus IV | B. cereus G9241 | ND | 5.2 ± 0.7 | 63.4 ± 2.1 | 9.5 ± 1.9 | 21.7 ± 1.0 | ND |
| B. cereus 03BB87 | ND | 2.5 ± 0.9 | 57.4 ± 9.2 | 11.4 ± 6.6 | 28.0 ± 3.2 | ND | |
| 2/Tolworthi | B. cereus ATCC 14579 | ND | 27.7 ± 2.2 | ND | 14.5 ± 4.5 | 45.2 ± 2.5 | 12.2 ± 3.1 |
| 2/Kurstaki | B. thuringiensis ATCC 33679 | ND | 55 | ND | 7.2 | 30 | 7.7 |
| 2/Sotto | B. thuringiensis ATCC 35646 | ND | 20 | ND | 15 | 49 | 17 |
a
Values are given as the percentage of total carbohydrate before HF treatment. For the strains B. anthracis Sterne 34F2 and B. cereus ATCC 10987, a high Glc content was occasionally observed in cell wall preparations. The sugar compositions given here are from cell wall preparations confirmed in independent culturing experiments. ND, none detected (i.e., <0.5%). N-acetylmuramic acid was also detected in the cell wall preparations but was not quantified. For the MLST classifications, see Table 1.
b
atxA deletion mutant of 7702.